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猪是重要的农业经济动物，也是生物医学研究的理想动物模型之一。自2013年首次将成簇的规律间隔短回文重复序列(CRISPR/Cas9)系统应用于哺乳动物以来，基因编辑猪在农业育种、医学研究、人源化器官生产和临床疾病治疗等多个领域取得突破性进展。文章围绕基因编辑猪的研究现状，对CRISPR/Cas9系统在猪遗传育种、人类疾病模型建立和异种器官移植等方面的应用进行综述，以期为我国养猪业健康发展和生物医药研究提供参考。
Abstract：Pigs are not only important agricultural economic animals, but also ideal animal models for biomedical research. Since the initial application of the CRISPR/Cas9 CRISPR/Cas9(clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9, CRISPR/Cas9) system in mammals in 2013, gene-edited pigs have made breakthroughs in a number of fields including agricultural breeding, medical research, humanized organ production and clinical disease treatment. In order to provide a reference for the healthy development of animal husbandry and biomedical research in China, this paper provides a review on the application of the CRISPR/Cas9 system in genetic breeding, human disease modeling and xenotransplantation in pigs under the current status of research on gene-edited pigs.
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基本信息:
DOI：10.19848/j.cnki.ISSN1005-2739.2021.12.0001
中图分类号:S828
引用信息:
[1]于海颖,路永强,张鲁,等.CRISPR/Cas9系统在基因编辑猪生产中的应用[J].黑龙江动物繁殖,2022,30(01):34-40.DOI:10.19848/j.cnki.ISSN1005-2739.2021.12.0001.
基金信息:
国家自然科学基金重点项目(31830091)
2022-01-20
2022
2022-04-06
2022
1
2022-01-15
2022-01-15