添加植物抗冻蛋白提高猪胚胎冷冻保存成活率的初步试验Additive of AFPGs in Enhencing Survival Rate of Frozen Swine Embryos
费云标,魏令波,高素琴,朱化彬,罗应荣,张之宣
摘要(Abstract)：
本试验采集7天猪扩张囊胚和孵化后囊胚,在1.5M甘油冷冻液中添加二种浓度(0.5mg/ml,5mg/ml)植物抗冻蛋白(AFGPs),用慢速降温方法冷冻。猪胚胎冷冻-解冻后,体外培养12小时,发育率分别为25％(1/4)和80％(4/5);24小时发育率分别为0％(0/4)和40％(2/5),孵化囊胚率分别为0％(0/5)和20％(1/5),而对照组冷冻-解冻后培养发育率12小时和24小时均为0％(0/4)。试验表明,在冷冻液中添加一定浓度的植物抗冻蛋白,可提高猪胚胎冷冻-解冻后的成活率。
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作者(Author): 费云标,魏令波,高素琴,朱化彬,罗应荣,张之宣
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