赵丙辰,张宇,李澍祺,华思锐,白素英

• 1:东北林业大学野生动物与自然保护地学院

摘要(Abstract)：

试验旨在探究麝鼠前列腺组织中褪黑素受体1(Mt1)基因的季节性表达差异，并解析其RNA及蛋白结构特征与功能。采集繁殖期与非繁殖期麝鼠前列腺组织，通过荧光定量PCR检测Mt1的mRNA表达水平，结合mRNA二级结构预测、蛋白理化性质分析、跨膜结构及蛋白互作预测，揭示其在季节性繁殖中的潜在作用机制。结果表明：非繁殖期麝鼠前列腺中Mt1的mRNA表达量极显著高于繁殖期（P<0.000 1），表明其可能参与非繁殖期组织稳态维持。mRNA结构预测显示，Mt1基因RNA具有多茎环构象（最小自由能MFE=-1 444.7 kJ/mol）;Mt1蛋白含7次跨膜螺旋（覆盖161个氨基酸），理论等电点pI=9.45，疏水性强，胞外结构域含12个半胱氨酸残基，可能通过二硫键稳定构象。SMART结构域预测显示，其属于Srsx亚家族，与精子发生相关蛋白功能特征相符。互作网络预测表明，Mt1蛋白的互作网络高度依赖G蛋白异源三聚体（Gαi/Gβγ）,Gpr50可能作为调控因子参与其中，推测非繁殖期Mt1的高表达可能通过与Gαi亚基、Gβγ复合物及潜在调控受体Gpr50相互作用，调控前列腺代谢状态，从而适应长日照环境下的生殖抑制需求。综上，麝鼠前列腺中Mt1基因的极显著季节性表达通过其多茎环RNA结构、跨膜蛋白特性及G蛋白互作网络调控前列腺代谢，介导长日照环境下季节性生殖抑制的分子适应机制。

关键词(KeyWords)： 麝鼠;前列腺;季节性繁殖;Mt1基因;生物信息学分析

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵丙辰,张宇,李澍祺,华思锐,白素英

DOI: 10.19848/j.cnki.ISSN1005-2739.2025.06.0007

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